KMID : 1100720210410010108
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Annals of Laboratory Medicine 2021 Volume.41 No. 1 p.108 ~ p.113
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A Sensitive and Specific Liquid Chromatography-Tandem Mass Spectrometry Assay for Simultaneous Quantification of Salivary Melatonin and Cortisol: Development and Comparison With Immunoassays
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Shin Sung-Hwan
Oh Hyeon-Ju Park Hea-Ree Joo Eun-Yeon Lee Soo-Youn
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Abstract
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Melatonin and cortisol are clinically important for diagnosing sleep and mood disorders. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of salivary melatonin and cortisol concentrations according to the Clinical and Laboratory Standards Institute guidelines. Additionally, we compared the LC-MS/MS assay with immunoassays, ELISA (Direct Salivary Melatonin Elisa EK-DSM, Buhlmann Laboratories AG, Schonenbuch, Switzerland) and electrochemiluminescence immunoassay (Cortisol II, Roche, Mannheim, Germany), using 121 saliva samples. The LC-MS/MS assay exhibited good performance in terms of linearity, precision, accuracy, limit of detection, lower limit of quantification, extraction recovery, carry-over, and matrix effect. The LC-MS/MS assay and immunoassays showed strong correlation (Pearson¡¯s r=0.910 for melatonin, r=0.955 for cortisol), but demonstrated a significant mean bias of 23.2% (range 54.0?143.7%) for melatonin and 48.9% (range 59.7?184.7%) for cortisol. Our LC-MS/MS assay provided more sensitive and reliable salivary melatonin and cortisol quantification results compared with immunoassays.
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KEYWORD
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Melatonin, Cortisol, Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Immunoassay, Saliva, Performance
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